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CuCDs and 5-Fu@CuCDs promote intracellular copper uptake and <t>mitochondrial</t> co-localization in AMC-HC-8 cells (A) CuprosGreen staining (green) shows intracellular copper signals in AMC-HC-8 cells after treatment with 5-Fu, CuCDs, or 5-Fu@CuCDs. Nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 50 μm. (B) Co-localization of intracellular copper signals and mitochondria in AMC-HC-8 cells under different treatments, as shown by CuprosGreen (green) staining and <t>MitoTracker</t> (red) labeling. Nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 20 μm.
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CuCDs and 5-Fu@CuCDs promote intracellular copper uptake and <t>mitochondrial</t> co-localization in AMC-HC-8 cells (A) CuprosGreen staining (green) shows intracellular copper signals in AMC-HC-8 cells after treatment with 5-Fu, CuCDs, or 5-Fu@CuCDs. Nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 50 μm. (B) Co-localization of intracellular copper signals and mitochondria in AMC-HC-8 cells under different treatments, as shown by CuprosGreen (green) staining and <t>MitoTracker</t> (red) labeling. Nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 20 μm.
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CuCDs and 5-Fu@CuCDs promote intracellular copper uptake and mitochondrial co-localization in AMC-HC-8 cells (A) CuprosGreen staining (green) shows intracellular copper signals in AMC-HC-8 cells after treatment with 5-Fu, CuCDs, or 5-Fu@CuCDs. Nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 50 μm. (B) Co-localization of intracellular copper signals and mitochondria in AMC-HC-8 cells under different treatments, as shown by CuprosGreen (green) staining and MitoTracker (red) labeling. Nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 20 μm.

Journal: iScience

Article Title: Tumor microenvironment responsive copper-doped carbon dots for synergistic chemotherapy and cuproptosis in laryngeal cancer

doi: 10.1016/j.isci.2026.115818

Figure Lengend Snippet: CuCDs and 5-Fu@CuCDs promote intracellular copper uptake and mitochondrial co-localization in AMC-HC-8 cells (A) CuprosGreen staining (green) shows intracellular copper signals in AMC-HC-8 cells after treatment with 5-Fu, CuCDs, or 5-Fu@CuCDs. Nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 50 μm. (B) Co-localization of intracellular copper signals and mitochondria in AMC-HC-8 cells under different treatments, as shown by CuprosGreen (green) staining and MitoTracker (red) labeling. Nuclei were counterstained with Hoechst 33342 (blue). Scale bars, 20 μm.

Article Snippet: Subsequently, co-localization imaging was performed using CuprosGreen together with the mitochondrial tracker MitoTracker Deep Red FM (MedChemExpress, USA).

Techniques: Staining, Labeling